anti-mouse cd122 tm-beta 1  (Bio X Cell)

Journal: PLoS ONE

Article Title: Clonal Expansions of CD8 + T Cells with IL-10 Secreting Capacity Occur during Chronic Mycobacterium tuberculosis Infection

doi: 10.1371/journal.pone.0058612

Figure Lengend Snippet: C57BL/6 and CBA/J mice were infected with an aerosolized dose of Mtb and at various timepoints post-infection lungs were removed and processed for flow cytometry. Absolute numbers of IFN-γ + CD4 + ( a ) or CD8 + ( b ) T cells after 4 hr ex vivo stimulation with anti-CD3/CD28/GolgiSTOP, with representative flow plots at day 150 post-infection. Absolute numbers of CD8 + T cells expressing CD69 ( c ), Tim3 ( d ), or PD-1 ( e ) after Mtb infection. ( f ) Absolute number of CD8 + T cells expressing both PD-1 and CD122. Data representative of at least two independent experiments with 4 mice per group per timepoint. * p<0.05, ** p<0.01, *** p<0.001 as obtained by Student's t test. ( c, d ) + p<0.05, ++ p<0.01, +++ p<0.001 obtained by two-way analysis of variance comparing only CBA/J mice across all timepoints.

Article Snippet: Specific antibodies and isotype controls were purchased from BD Biosciences: PerCP-Cy5.5 anti-CD3ε (145-2C11), allophycocyanin-Cy7 anti-CD4 + (GK1.5), PE-Cy7 anti-CD8 + (53–6.7), PerCP-Cy5.5 anti-CD8 + (53–6.7), PE-Cy7 anti-IFN-γ (XMG1.2), PE anti-PD-1 (J43), FITC anti-CD122 (TM-Beta 1), and FITC Vβ screening kit.

Techniques: Infection, Flow Cytometry, Ex Vivo, Expressing